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Step 1

A cidal agent means that it causes death of an organism. A static agent does not directly kill the organism but prevents its growth. They are reversible that if removed from the system microorganisms can start to grow again.

 

 

 

 

Step 2

 

An antimicrobial agent can be tested for its property by growing the microorganism in the presence of the agent. First it should be established that the antimicrobial agent is actually antimicrobial. Inoculating the microorganism on solid media plates, which contain the antimicrobial, could test this. Several different concentrations should be tested, as one concentration may not be enough to affect the organism.

Step 3

 

 

To determine whether the agent is cidal or static, the agent could be added to bacteria growing in a liquid culture. The agent treated culture can be centrifuged down and washed several times with liquid media that does not contain the antimicrobial agent. This should remove the agent from the system. The culture can then be plated on solid agar or re-inoculated into liquid media without the antimicrobial agent. If the microorganism grows then it was a static agent. If the microorganism does not grow then it was a cidal agent.

Step 4

 

 

A disinfectant involves the complete removal of all microorganisms that cause disease from objects that are typically inanimate. An antiseptic kills inhibits pathogens that are in living tissue. Antiseptics are generally less toxic than disinfectants. If the antimicrobial agent does not harm living tissue then it would be acceptable to use as an antiseptic



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